Cell Name: Stable-overexpressing human APP in SH-SY5Y cell line
Catalogue No.: CGSC-M25881

Modified Gene: hAPP

Gene ID: 351

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_000484.4

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Tissue origin: Brain

Cell morphology: Epithelial cells

Characteristics: Semi-adherent

Culture media: MEM/F12＋15% FBS＋1% P/S

Passage ratio: 1:3-1:4, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative