Cell Name: Stable-overexpressing human HLF in HCT116 cell line
Catalogue No.: CGSC-M25781

Modified Gene: hHLF

Gene ID: 3131

Modification Type: Overexpression

Method: LV

Transcript: NM_002126.5

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus：Homo sapiens

Tissue origin：Colon

Cellular morphology：Epithelioid

Characteristics：Adherent

Culture media: McCoy's 5A+10%FBS+1.0 ug/mL Puromycin+1%P/S

Passage ratio: 1:3-1:8 , change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO_{2 ,} 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative