Cell Name: Stable-overexpressing human MDR1 in MDCK.2 cell line
Catalogue No.: CGSC-M25771

Modified Gene: hMDR1

Gene ID: 5243

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_001348946.2

Resistance gene: Puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Canine

Tissue origin: Canine kidney

Cell morphology: Epithelioid

Characteristics: Adherent

Culture media: DMEM-H＋10% FBS＋1% P/S

Passage ratio: 1:2-1:3, change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative