Cell Name: Stable-overexpressing human SLC47A1 in MDA-MB-436 cell line
Cell Synonyms: CGSC-M25731

Modified Gene: hBRCA1

Gene ID: 672

Modification Type: Overexpression

Method: LV

Transcript: [NM_007296.2](ns):T2A:Bsd

Resistance gene: Bsd

Fluorescent gene: —

Clone Type: Poolcells/MixClone

Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Tissue origin: Mammary gland   

Cell morphology: Polymorphic

Characteristics: Adherent

Culture media: L-15+20% FBS+0.01mg/ml Insulin＋1% P/S

Passage ratio: 1:2-1:3, change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: air , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative