HyCyte cell line: Stable-overexpressing mouse nur77 in MC3T3-E1 cell line

Catalogue No.: CGSC-M25641

Gene: mnur77

Gene ID: 15370

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_010444.3

Resistance gene: puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Mus musculus

Tissue origin: Calvaria

Cell morphology: Fibroblast-like

Characteristics: Adherent

Culture media: MEMα＋10% FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Passage ratio: 1:4-1:6, change the media every 2-3 days.

Passage period: 24-32 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative