Cell Name: Stable-overexpressing mouse Herpud1 in 4T1 cell line

Catalogue No.: CGSC-M25571

Modified Gene: mHerpud1

Gene ID: 64209

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_022331.2

Resistance gene: puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G705).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative