Cell Name: Stable-overexpressing human GPER1 in 293T cell line
Catalogue No.: CGSC-M24291

Modified Gene: GPER1

Gene ID: 2852

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_001039966.2]

Resistance gene: puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Tissue origin: Embryonic kidney Disease

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%P/S

Passage ratio: 1:4-1:8, change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative