Cell Name: Stable-overexpressing human PIK3R1 in MKN-45 cell line

Catalogue No.: CGSC-M24241

Modified Gene: hPIK3R1

Gene ID: 5295

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: [NM_181523.3]/3xFLAG

Resistance gene: puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Cell morphology: Lymphoblastoid

Characteristics: Semi-adherent

Culture media: RPMI-1640＋10% FBS＋1%P/S

Passage ratio: 1:3-1:4, change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative