Cell Name: Stable-overexpressing human FASN in 786-O cell line
Cell Synonyms: CGSC-M23201

Modified Gene: hFASN

Gene ID: 2194

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: NM_004104.5

Resistance gene: puro

Fluorescent gene: EGFP

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: RPMI-1640＋10% FBS＋1% P/S

Passage ratio: 1:3-1:4, change the media every 2-3 days.

Passage period: 24-48 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative