Cell name: Stable-overexpressing human MDH2 in MDA-MB-231 cell line

Catalogue No.: CGSC-M22551

Modified Gene: hMDH2

Gene ID: 4191

Modification Type: Overexpression

Method: Piggy-BAC

Transcript: [NM_005918.4]/FLAG

Resistance gene: puro

Fluorescent gene: —

Clone Type: Poolcells

Cellular Identification: PCR, Sequencing

Genus: Homo sapiens

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative