HyCyte cell line: HyCyte HEK293 hFBN1_c.7819+1G＞A cell line

Catalogue No.: CGMU-M2278

Parental：HEK293

Gene: hFBN1

Gene ID: 2200

Genotype：Homozygous

Mutation：   c.7819+1G＞A

Species：Homo sapiens, human

Passage: 9

Doubling time: 24--48h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G190).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).