HyCyte cell line: HyCyte hCMEC/D3 hKSR2_rs12822146 cell line

Catalogue No.: CGMU-M2277

Parental: hCMEC/D3

Gene:hKSR2

Gene ID: 283455

Genotype：Homozygous

Mutation：rs12822146

Species：Homo sapiens, human

Passage: 9

Doubling time: 36--54h

Growth Properties：Adherent

Recommended culture media:Immortalized Human Brain Microvascular Endothelial Cell Basal Medium supplemented with 5% FBS, 1% Endothelial Cell Growth Supplement, and 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G576).

Subculture:   Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:   60% basal medium + 30% FBS + 10% DMSO