HyCyte cell line: HyCyte U2OS hDARS2_ p.S242A_c.725A>G_c.724G>C cell line

Catalogue No.: CGMU-M2188

Parental: U2OS

Gene:hDARS2

Gene ID: 55157

Genotype：Homozygous

Mutation：   p.S242A_c.725A>G_c.724G>C

Species：Homo sapiens, human

Passage: 9

Doubling time: 18--24h

Growth Properties：Adherent

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G365).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Quality test: Bacteria, fungi and mycoplasma negative