HyCyte cell line: HyCyte SW48 hNRAS_p.Q61H_c.183A＞C cell line

Catalogue No.:CGMU-M2274

Parental: SW48

Gene: hNRAS

Gene ID: 4893

Genotype：Homozygous

Mutation：p.Q61H_c.183A＞C

Species：Homo sapiens, human

Passage: 9

Doubling time: 60--72h

Growth Properties：Adherent

Recommended culture media:

Leibovitz L-15 Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G425).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 0% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:L-15+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)