HyCyte cell line: HyCyte GM12878 hSLC26A4_ p.N392Y_c.1174A>T cell line

Catalogue No.: CGMU-M2185

Parental: GM12878

Gene: hSLC26A4

Gene ID: 5172

Genotype：Homozygous

Mutation：   p.N392Y_c.1174A>T

Species：Homo sapiens, human

Passage: 9

Doubling time: 24--48h

Growth Properties：Suspension

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G407).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Negative for bacteria, fungi, and mycoplasma detection