HyCyte cell line: HyCyte HCT 116 hJAK2_ p.V617F_c.1849G>T cell line

Catalogue No.: CGMU-M2183

Parental: HCT 116

Gene: hJAK2

Gene ID: 3717

Genotype：Heterozygote

Mutation： p.V617F_c.1849G>T

Species：Homo sapiens, human

Passage: 9

Doubling time: 24-36 h

Growth Properties：Adherent

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G185）

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:8

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Quality test: Bacteria, fungi and mycoplasma negative