HyCyte cell line: HyCyte PC-9 hEGFR_p.L704N cell line

Catalogue No.:CGMU-M2181

Parental: PC-9

Gene:hEGFR

Gene ID: 1956

Genotype：Homozygous

Mutation：p.L704N

Species：Homo sapiens, human

Passage: 9

Doubling time: 24--32h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G720).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative