HyCyte cell line: HyCyte C2C12 mItgb6_p.F400V_c.1198T>G cell line

Catalogue No.: CGMU-M2180

Parental: C2C12

Gene:mItgb6

Gene ID:16420

Genotype：Homozygous

Mutation： p.F400V_c.1198T>G

Species：mouse

Passage: 9

Doubling time: 24--36h

Growth Properties：Adherent

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G365).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Quality test: Bacteria, fungi and mycoplasma negative