HyCyte cell line: HyCyte MCF-7 hESR1_p.Y537S_c.1610A>C cell line

Catalogue No.: CGMU-M2179

Parental: MCF-7

Gene: hESR1

Gene ID: 2099

Genotype：Homozygous

Mutation：p.Y537S_c.1610A>C

Species：Homo sapiens, human

Passage: 9

Doubling time: 24--32h

Growth Properties：Adherent

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,1%NEAA, 10 ug/mL Human recombinant insulin,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G247).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative