HyCyte cell line: HyCyte THP-1 hLACC1_p.V254I_c.760G＞A cell line

Catalogue No.: CGMU-M2255

Parental: THP-1

Gene:hLACC1

Gene ID: 144811

Genotype：Homozygous

Mutation：p.V254I_c.760G＞A

Species：Homo sapiens, human

Passage: 9

Doubling time: 24--36h

Growth Properties：Suspension

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS,0.05 mM β-mercaptoethanol, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G361).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).