HyCyte cell line: HyCyte MC-38 mKras_p.G12D_c.35G>A cell line

Catalogue No.: CGMU-M2178

Parental: MC-38

Gene:mKras

Gene ID: 16653

Genotype：Homozygous

Mutation：   p.G12D_c.35G>A

Species：mouse

Passage: 9

Doubling time: 18--24h

Growth Properties：Adherent

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G790).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative