HyCyte cell line: HyCyte Huh-7 hACSM5_p.P352R_c.1055C>G cell line

Catalogue No.: CGMU-M2239

Parental: Huh-7

Gene: hACSM5

Gene ID: 54988

Genotype：Homozygous

Mutation：p.P352R_c.1055C>G

Species：Human

Passage: 9

Doubling time: 36-48 h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G217).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative