HyCyte cell line: HyCyte THP-1 hIRF8_c.1278/insT/c.1279 cell line

Catalogue No.: CGMU-M2236

Parental: THP-1

Gene: hIRF8

Gene ID: 3394

Genotype：Homozygous

Mutation：c.1278/insT/c.1279

Species：Human

Passage: 9

Doubling time: 24-36 h

Growth Properties：Suspension

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS,0.05 mM β-mercaptoethanol, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G361).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative