HyCyte cell line: HyCyte HEK293T hTMED2_p.L185F_c.555G>C   cell line

Catalogue No.: CGMU-M2235

Parental: HEK293T

Gene: hTMED2

Gene ID: 10959

Genotype：Homozygous

Mutation：p.L185F_c.555G>C

Species：Human

Passage: 9

Doubling time: 24-48 h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G101）

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Quality test: Bacteria, fungi and mycoplasma negative