HyCyte cell line: HyCyte HK-2 hFOXI1_p.N161Y_c.481A>T cell line

Catalogue No.: CGMU-M2223

Parental: HK-2

Gene: hFOXI1

Gene ID: 2299

Genotype：Homozygous

Mutation：p.N161Y_c.481A>T

Species：Human

Passage: 9

Doubling time: 24-36 h

Growth Properties：Adherent

Recommended culture media:

Minimum Essential Medium (MEM)   supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G400）

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Quality test: Bacteria, fungi and mycoplasma negative