HyCyte cell line: HyCyte Hep-G2 hPCF11_p.S590R_c.1770T>A cell line

Catalogue No.: CGMU-M2219

Parental: Hep-G2

Gene: hPCF11

Gene ID: 51585

Genotype：Homozygous

Mutation：p.S590R_c.1770T>A

Species：Human

Passage: 9

Doubling time: 60-72 h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G196).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative