HyCyte cell line: HyCyte SK-MEL-1 hBRD4_c.1213-1G>T cell line

Catalogue No.: CGMU-M2214

Parental: SK-MEL-1

Gene: hBRD4

Gene ID: 23476

Genotype：Homozygous

Mutation：c.1213-1G>T

Species：Human

Passage: 9

Doubling time: 48-60 h

Growth Properties：Suspension

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G461).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative