HyCyte cell line: HyCyte LLC mEgfr_p.L860R_c.2579T>G cell line

Catalogue No.: CGMU-M2212

Parental: LLC

Gene: mEgfr

Gene ID: 13649

Genotype：Homozygous

Mutation：p.L860R_c.2579T>G

Species：Mouse

Passage: 9

Doubling time: 18-24 h

Growth Properties：Semi-adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)    supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G742）

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Quality test: Bacteria, fungi and mycoplasma negative