HyCyte cell line: HyCyte Jurkat hLCK_p.C476A_c.1426T>G_c.1427G>C cell line

Catalogue No.: CGMU-M2210

Parental: Jurkat

Gene: hLCK

Gene ID: 3932

Genotype：Homozygous

Mutation：p.C476A_c.1426T>G_c.1427G>C

Species：Human

Passage: 9

Doubling time: 36-48 h

Growth Properties：Suspension

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G225).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative