HyCyte cell line: HyCyte A-549 hRB1CC1_p.R474H_c.1421G>A_c.1422C>T cell line

Catalogue No.: CGMU-M2206

Parental: A-549

Gene: hRB1CC1

Gene ID: 9821

Genotype：Homozygous

Mutation：p.R474H_c.1421G>A_c.1422C>T

Species：Human

Passage: 9

Doubling time: 36-48 h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12）   supplemented with 10 % FBS,, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G116）

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Quality test: Bacteria, fungi and mycoplasma negative