HyCyte cell line: HyCyte Myc-CaP mTrp53_p.R245Q_c.734G>A_c.735C>G cell line

Catalogue No.: CGMU-M2203

Parental: Myc-CaP

Gene: mTrp53

Gene ID: 22059

Genotype：Homozygous

Mutation：p.R245Q_c.734G>A_c.735C>G

Species：Mouse

Passage: 9

Doubling time: 18-24 h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G796).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative