HyCyte cell line: HyCyte HT22   mSirt2_p.S368D_c.1102T>G_c.1103C>A cell line

Parental: HT22

Catalogue No.: CGMU-M2197

Gene: mSirt2

Gene ID: 64383

Passage: 9

Doubling time: 24-48 h

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS,,100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G821).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative