HyCyte cell line: HyCyte TMD8 hBTK_p.L528W_c.1583T>G cell line

Catalogue No.: CGMU-M2176

Parental: hBTK

Gene: mSirt2

Gene ID: 695

Genotype：Homozygous

Mutation：p.L528W_c.1583T>G

Species：Human

Passage: 9

Doubling time: 36-48 h

Growth Properties：Adherent

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G581).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative