HyCyte cell line: HyCyte THP-1 hKDM6A_p.A694T_c.2080G>A cell line

Parental: THP-1

Catalogue No.: CGMU-M2174

Gene: hKDM6A

Gene ID: 7403

Passage: 9

Doubling time: 24-48 h

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS,0.05 mM β-mercaptoethanol, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G361).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative