HyCyte cell line: HyCyte T24 hADAR_p.E912A_c.2735A>C_c.2736A>C cell line

Parental: T24

Catalogue No.: CGMU-M2163

Gene: hADAR

Gene ID: 103

Passage: 9

Doubling time: 24-48 h

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G352).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Quality test: Bacteria, fungi and mycoplasma negative