Cell name: Pole point mutant CT26 cell line
Abbreviation:CT26-MU-Pole
Product Code:CGMU-M2148
Modified Gene: Pole_p.V411L_c.1231G>C
Gene ID: 18973
Modification Type: Point mutation
Transduction/Transfection: protein electroporation
Clone Type: homozygote
Cellular Identification: PCR, Sequencing
Genus: Mouse
Tissue origin: colon
Cell morphology: Embryonic kidney Disease
Characteristics: Adherent
Culture media: RPMI-1640+10%FBS+1%P/S
Passage ratio: 1:3-1:6, change the media every 2-3 days.
Passage period: 48-72 h
Culture conditions: 95% air + 5% CO2 , 37℃
Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage
Quality test: bacteria, fungi and mycoplasma negative