Cell name：PIK3R1 point mutant HepG2 cell line

Abbreviation：HepG2-MU-PIK3R1

Catalogue No.: CGMU-M2146

Modified Gene: GUCY2D_ p.R838S_c.2512C>A

Gene ID: 5295

Modification Type: Point mutation

Transduction/Transfection: Protein electroporation

Clone Type: homozygote

Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+1%P/S

Passage ratio: 1:2-1:4, change the media every 2-3 days.

Passage period: 72-96 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative