Cell name：GUCY2D point mutant ARPE-19 cell line

Abbreviation：ARPE-19-MU-GUCY2D

Catalogue No.: CGMU-M2139

Modified Gene: GUCY2D_ p.R838S_c.2512C>A

Gene ID: 3000

Modification Type: Point mutation

Transduction/Transfection: Protein electroporation

Clone Type: homozygote

Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DF-12+10%FBS+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative