Cell name：Bate-catenin point mutant Hepa1-6 cell line

Abbreviation：Hepa1-6-MU-bate-catenin

Catalogue No.: CGMU-M2137

Modified Gene: bate-catenin_p.Y142E_c.424T>G_c.426T>G

Gene ID: 12387

Modification Type: Point mutation

Transduction/Transfection: Protein electroporation

Clone Type: homozygote

Cellular Identification: PCR, Sequencing

Genus: Mouse

Cell morphology: Epithelial cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative