Cell name： Chrne1 point mutant C2C12 cell line

Abbreviation：C2C12-MU-Chrna1

Modified Gene：Chrna1_p.F253V_c.757T>G

Gene ID：11435

Modification Type: Point mutation

Transduction/Transfection: Plasmid electroporation

Clone Type：Homozygote

Cellular Identification: PCR, Sequencing

Genus: Mouse

Tissue origin: Muscle

Morphology: Myoblast-like cell   

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative