Cell name：RERE point mutant U251 cell line

Abbreviation：U251-MU-RERE

Product Code：CGMU-M2121

Modified Gene：RERE_p.P1244fs*13_c.3732delC

Gene ID: 473

Modification Type: Point mutation

Transduction/Transfection: Plasmid electroporation

Clone Type: Homozygote

Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Cell morphology: Fibroblast Cells

Characteristics: Adherent

Culture media: DMEM-H+10%FBS+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 48-72 h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative