Cell name：TLR8 point mutant THP-1 cell line

Abbreviation：THP1-MU-TLR8

Catalogue No.: CGMU-M2120

Modified Gene: TLR8_p.V101 L_c.301G>C

Gene ID: 51311

Modification Type: Point mutation

Transduction/ Transfection: Protein electroporation

Clone Type: homozygote

Cellular Identification: PCR, Sequencing

Genus: homo sapiens

Tissue origin: Peripheral blood

Characteristics: Suspension cell

Culture media: RPMI-1640+10%FBS+0.05 mM β-mercaptoethanol+1%P/S

Passage ratio: 1:3-1:6, change the media every 2-3 days.

Passage period: 24-48h

Culture conditions: 95% air + 5% CO₂ , 37℃

Freezing conditions: 60% basal medium+30% FBS+10% DMSO, liquid nitrogen storage

Quality test: bacteria, fungi and mycoplasma negative