HyCyte Cell Line:HyCyte MEG-01 KO-hCST7

Parental: MEG-01

Catalogue No.: CGKO-M3515

Species:Homo sapiens, human

Gene: hCST7

Gene ID: 8530

Passage: 9

Doubling time: 36--48h

Growth Properties: Semi-adherent

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G255).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media:RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: mTrpv4 (-/-)