HyCyte Cell Line: HyCyte Nthy-ori 3-1 KO-hCCL5

Parental: Nthy-ori 3-1

Catalogue No.: CGKO-M3354

Species: human

Gene: hCCL5

Gene ID: 6352

Passage: 9

Doubling time: 24-48 h

Growth Properties: Semi-adherent

Recommended culture media:RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G472).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hCCL5 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative