HyCyte Cell Line: HyCyte MDA-MB-436 KO-hARTN

Parental: MDA-MB-436

Catalogue No.: CGKO-M3186

Species: Human

Gene: hARTN

Gene ID: 9048

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Leibovitz L-15 Medium   supplemented with 20 % FBS,10 ug/mL Human recombinant insulin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G251).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 0% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: L-15+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hARTN (-/-)

Quality test: Bacteria, fungi and mycoplasma negative