HyCyte Cell Line: HyCyte MDA-MB-231 KO-hRASAL2

Parental: MDA-MB-231

Catalogue No.: CGKO-M2723

Species: Human

Gene: hRASAL2

Gene ID: 9462

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G453).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hRASAL2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative