HyCyte Cell Line: HyCyte HK-2 [Human kidney] KO-hKDM5A

Parental: HK-2 [Human kidney]

Catalogue No.: CGKO-M2654

Species: Human

Gene: hKDM5A

Gene ID: 5927

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G400).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hKDM5A (-/-)

Quality test: Bacteria, fungi and mycoplasma negative