HyCyte Cell Line: HyCyte MCF-7 KO-hINSR

Parental: MCF-7

Catalogue No.: CGKO-M2533

Species: Human

Gene: hINSR

Gene ID: 3643

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,1%NEAA,10 ug/mL Human recombinant insulin,100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G247).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hINSR (-/-)

Quality test: Bacteria, fungi and mycoplasma negative