HyCyte Cell Line: HyCyte A-549 KO-hMUC4

Parental: A-549

Catalogue No.: CGKO-M2527

Species: Human

Gene: hMUC4

Gene ID: 4585

Passage: 9

Doubling time: 48-72h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G116).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hMUC4(-/-)

Quality test: Bacteria, fungi and mycoplasma negative