HyCyte Cell Line: HyCyte HK-2 [Human kidney] KO-hNPHP3

Parental: HK-2 [Human kidney]

Catalogue No.: CGKO-M2504

Species: Human

Gene: hNPHP3

Gene ID: 27031

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G400).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hNPHP3 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative